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. 2019 Dec 26;12(1):1702263. doi: 10.1080/19420862.2019.1702263

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of loop size between dimensions on ²D SEC separation. (a) Half-height width of monomer peak in ²D SEC. (b) Resolution between HMW and monomer species in ²D SEC. For the resolution calculation, widths of peaks were calculated using tangent line method, because half-height width could not be measured for the HMW species peak in some cases. Heart cuts for each loop size were timed to optimize recovery for ²D SEC. (c) Recovery of material in ²D SEC compared to 1D SEC. In all graphs, error bars show 1 standard deviation.