Table 2.

Linear regressions of 1D SEC and 2D ProA-SEC methods in response to changing %HMW samples. Standard error (SE) for each value is shown in parenthesis.

	Elution Conditions	Slope (SE)	Y-intercept (SE)	R2
mAb1	2D pH 3.0	0.45 (0.02)	0.11 (0.10)	0.9961
	2D pH 2.25, 0.5 mL/min	0.82 (0.01)	0.36 (0.09)	0.9991
mAb3	2D pH 3.0	0.65 (0.01)	−0.10 (0.08)	0.9986
	2D pH 2.8, 0.5 mL/min	0.77 (0.01)	2.33 (0.05)	0.9995