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Hyperosmotic NaCl and sucrose stress cause chromatin congelation. HL-60/S4 cells incubated in the presence of 300 mM sucrose (a) or of 150 mM NaCl (b), immunostained with mAb PL2-6 (anti-epichromatin, red) and DAPI (cyan). Each panel contains one interphase nucleus and one cluster of congealed mitotic chromosomes. Magnification bar, 5 µm.
