Table 1.
Viability of HL-60/S4 cells in tissue culture medium + 300 mM sucrose. Each sample was measured in triplicate. The mean values are shown. Viability and Membrane Polarization were measured using separate kits, with the Guava easyCyte single-cell analysis system (EMD Millipore Corp.).
| Viability |
Mitochondrial Polarization |
|||
|---|---|---|---|---|
| Time in 300 mM Sucrose | % Viable | % Dead | % Polarized | % Depolarized |
| 0 | 99.5 | 0.5 | 94.3 | 5.7 |
| 30 minutes | 99.3 | 0.7 | 94.1 | 5.8 |
| 1 hour | 99.6 | 0.4 | 91.7 | 8.3 |
| 2 hours | 97.6 | 2.4 | 87.6 | 12.4 |
| 3 hours | 95.2 | 4.8 | 83.0 | 17.0 |