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. 2020 Jan 15;13:351–359. doi: 10.2147/OTT.S200317

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© 2020 Liu et al.

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Effects of sh-SPOCD1 in SKOV3 and CAOV3 cells proliferation and metastasis. (A) Effect of knockdown SPOCD1 in SKOV3 and CAOV3 cells. (B, E) Effect of sh-SPOCD1on the cell proliferation rate in SKOV3 and CAOV3 cells expressing empty vector or sh-SPOCD1 and determined by CCK8 assay at the indicated time point, for P<0.05 (Vector vs sh-SPOCD1). (C, F) Effect of sh-SPOCD1 on colony formation. SKOV3 and CAOV3 cells with stable expression of vector or sh-SPOCD1 were seeded into six-well plates at 900 cells per well and cultured for 14 days, followed by crystal violet staining and colony counting. (D, G) Effect of sh-SPOCD1 on transwell assay. Data were analyzed as mean ± SD and for P<0.05 (Vector vs sh-SPOCD1).

Abbreviation: NC, normal control.