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. 2020 Jan 21;15(1):e0228072. doi: 10.1371/journal.pone.0228072

Fig 4. QRT-PCR results analyzed with a pair wise fixed reallocation randomization test.

Fig 4

CT values for mRNA area between exon 6–8 of the DMD gene were normalized to HPRT1 (house-keeping gene) as an internal control gene. Samples were analyzed using a pair wise fixed reallocation randomization test, excluding outliers with a Grubb’s test. Vertical boxes include standard error of the mean. RNA was extracted from each muscle and run in triplicate. All values were expressed as fold change compared to the normal dog values, which were also normalized to HPRT1 (dotted line). CT = cranial tibial; PL = peroneus longus; LDE = long digital extensor; *p<0.05; ***p<0.001.