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. 2020 Jan 15;11:342. doi: 10.3389/fnagi.2019.00342

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Copyright © 2020 Atluri, Tiwari, Rodriguez, Kaushik, Yndart, Kolishetti, Yatham and Nair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Congo red staining and amyloid-beta (Aβ40) ELISA for Aβ measurement: (Ai,ii) SH-APP cells were treated with 1 μM concentration of withaferin A and incubated for 48 h. Cells were stained with the Congo red stain. We have observed significantly reduced levels of amyloid-beta expression in WA-treated cells when compared to the DMSO-treated control cells. (B) SH-APP cells were cultured in six-well culture plates and after 48 h of incubation, cells were treated with withaferin A (1 μM). After 48 h of incubation, cell culture supernatant was analyzed for Aβ levels using Aβ40 ELISA and observed a significant reduction of Aβ40 levels upon WA treatment when compared to the DMSO-treated cells (^****p ≤ 0.0001).