Figure 2.

Podoplanin deletion alters long-term depression (LTD) selectively in the hippocampal DG. (A) Temporal courses of averaged slopes of fEPSP in CA3-Schaffer collateral-CA1 synapses (left panel) showed a strong main significant effect of time (****P < 0.0001, F_(76,1520) = 3.72, n = 11), no significant main effect of genotype (P = 0.6, F_(1,20) = 0.36, n = 11) and a significant interaction between time and genotype (***P = 0.0004, F_(76,1520) = 1.7). Corresponding bar graphs of the end-time points (right panel) showed no significant differences between Pdpn^+/+ and Pdpn^−/− (t₍₂₀₎ = 0.1124, P = 0.9, n = 11). (B) Temporal courses of averaged slopes of fEPSP in medial perforant pathway (MPP) of DG (left panel) showed a strong main significant effect of time (****P < 0.0001, F_(75,1275) = 27.87, n = 8–11), a moderate significant main effect of genotype (*P = 0.03, F_(1,17) = 5.99, n = 8–11) and a significant interaction between time and genotype (****P < 0.0001, F_(75,1275) = 1.88). Corresponding bar graphs of the end-time points (right panel) showed significant differences between Pdpn^+/+ and Pdpn^−/− (t₍₁₇₎ = 3.93, **P = 0.002, n = 8–11). Data are displayed as mean ± SEM.