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. 2020 Jan 15;10:1584. doi: 10.3389/fphys.2019.01584

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Copyright © 2020 Nagy, Pethő, Gáll, Zavaczki, Nyitrai, Posta, Zarjou, Agarwal, Balla and Balla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Zinc restores the viability of VSMCs in response to high Pi and PHI FG4592. VSMCs were cultured in growth medium in the presence or absence of PHI FG4592 (5 and 20 μmol/L) or in calcification medium with or without PHI FG4592 and zinc for 3, 6, and 10 days. (A–C). Cell viability was determined by MTT assay after (A) 3, (B) 6, and (C) 10 days. Statistical analysis was performed by one-way ANOVA test followed by Bonferroni correction. A value of p < 0.05 was considered significant. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.