Figure 5.

DGKαζDKO-exacerbated CD4⁺ T cell-mediated airway inflammation. The same WT and DKO OT2 T cell recipient mice intranasally injected with OVA in Figure 4 were utilized to collect BALF and lung tissues. (A) IFNγ and IL-17A concentrations in BALF. (B) Giemsa staining of fresh-harvested WT and DKO BALF from mice with or without OVA_323−339 challenge. (C) Leukocyte differentials in BALF calculated based on Giemsa staining. (D) H&E staining of thin lung sections. Data shown are representative of two independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 as determined by the Student t-test.