TABLE 1.

Genetic characteristics of 15 polymorphic simple-sequence repeat (SSR) loci in 59 Indian isolates of U. hordei.

Locus	Primer sequence (5′–3′)	Repeat motif	Amplicon size (bp)	Amplicon range	Ta (°C)	Polymorphism (%)	Na	He	PIC (%)
UHB3	TAGCCTTTGAGGTCGATGTAGG	(GTT)5	246	225–250	54	100	2	0.0997	0.0948
	TGGGTGTCTTTCAGATGAGTTG
UHB4	CAATTACTCGTCGTCCTCCTTC	(TCC)6	222	200–225	54	100	2	0.1349	0.1258
	AGCGTTCAGCGTAAGGTAGTTC
UHB5	GCTCGTCTACCTCTGCGATACT	(GGA)5	241	240–280	55	100	2	0.1769	0.1612
	TCTGCATCTCAATCAACCAATC
UHB6	AGACATGCACCGTAACAACAAC	(CGG)6	204	210–240	55	100	2	0.4767	0.3631
	TACCCTCCATACTCTTGTCCGT
UHB17	TCTTGTGGAGTCTGCTGTTGTT	(TGC)5	239	220–250	54	100	2	0.2688	0.2327
	GTAGCTTCAGGTCGCATCACTT
UHB 20	GGTTGTTGTCATAGGGGTTGTC	(TCG)4	259	260–520	54	100	2	0.4842	0.367
	TACCAGAACATGGGTTTCAGC
UHB21	AGGTCTGGTGTGAGTGTTGATG	(TGA)4	258	225–260	54	100	2	0.233	0.2058
	CTCCTCATTGTAGTGCGTGTGT
UHF 25	TACTTCTCCTCCTCCTCCTCCT GAACTCGCAAAGTGGTTTCTCT	(TATT)5	285	300–350	52	100	2	0.4904	0.3702
UHB 26	AGAGACCAAGTCGAATCCAAAG CCTTGCCTACTTCTCCCTACCT	(CAAGG)6	294	260–300	52	100	2	0.3866	0.3119
UHB 27	CATTTCAGTGTTGGACAAGCAT AGAGAGTTTCGTAGTTGGGCAG	(GTGTCA)4	251	200–250	52	100	2	0.2408	0.2118
UHB 28	CTAAGCATAAGGAGGCAACCAG	(TAAAA)5	271	280–850	54	100	2	0.4228	0.3334
	CGGAGTATTGGGAGTGAAATGT
UHB 30	GGTGATTGGAAGACCACAGAAT GTTTTGAACTCTCTGCTTTGGG	(AAGCCA)5	227	180–230	55	100	2	0.2726	0.2354
UHB 31	CACAAACACACACACACACACA	(GCTCCC)4	224	200–230	54	100	2	0.375	0.3047
	CTGAACAGTAAAGCCTGAAGGG
UHB 32	TCCTACATTGGGATGACTGATG GACTCGCTTCTTGTTCTTGGTT	(CAACGG)4	217	190–220	52	100	2	0.2008	0.1806
UHB 36	ATGAGGTCAAGAGTCAGCAACA ATTCGTCAAGATGCCTTTCACT	(GA)8	200	180–210	54	100	2	0.2149	0.1918

N_a, number of alleles; T_a, annealing temperature; H_e, expected heterozygosity; and PIC, polymorphism information content.