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. 2020 Jan 15;10:3115. doi: 10.3389/fimmu.2019.03115

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Copyright © 2020 Yeola, Ignatius Arokia Doss, Baillargeon, Akbar, Mailhot, Balood, Talbot, Anderson, Lacroix and Rangachari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological analysis of the CNS of 1C6 × Rag1^−/− mice that develop spontaneous EAE. Cerebellar (A–D) and spinal cord (E–H) lesions from an 18-weeks old male 1C6 × Rag1^−/− mouse that spontaneously developed paralytic disease. H&E staining (A,B,E,F) was used to identify inflammatory foci, and Luxol fast blue was used to detect myelin (C,D,G,H). Arrows indicate inflammatory damage. (A,C,E,G), 10× magnification. (B,D,F,H), 4× magnification. Scale bars, 100 μm. Representative of eight animals (6 female, 2 male).