Figure 5.

1C6 CD4⁺ T cells restrain CNS autoimmunity in 1C6 × Rag1^−/− mice. (A) Male 1C6 × Rag1^−/− mice were reconstituted (n = 5), or not (n = 5), with 2 × 10⁶ CD4⁺ T cells from unmanipulated male 1C6 mice. After 7 days, mice were actively immunized with MOG_[35−55] and were monitored for signs of EAE. Representative of 2 experiments. (B) Male 1C6 × Rag1^−/− mice were reconstituted (n = 5), or not (n = 5), with 2 × 10⁶ CD8⁺ T cells from unmanipulated male 1C6 mice. After 7 days, mice were actively immunized with MOG_[35−55] and were monitored daily for signs of EAE. *p < 0.05, two-tailed Mann-Whitney U test. ϕ indicates that all mice in the experimental group attained ethical endpoints. (C) Female 1C6 × Rag1^−/− mice were reconstituted (n = 4), or not (n = 4), with 2 × 10⁶ CD19⁺ B cells from unmanipulated 1C6 mice. After 7 days, mice were actively immunized with MOG_[35−55] and were monitored for signs of EAE. ϕ indicates that all mice in the experimental group attained ethical endpoints. *p < 0.05; #p < 0.01, two-tailed Mann-Whitney U test. ϕ indicates that all mice in the experimental group attained ethical endpoints. (D,E). Spleen (D) or CNS-infiltrating (E) CD4⁺ T cells were isolated from immunized 1C6 × Rag1^−/− mice when they reached ethical endpoints (n = 9), or from 1C6 × Rag1^−/− (reconstituted with 1C6 CD4⁺) that were sacrificed in parallel (n = 4). T cells were assessed ex vivo for the indicated cytokines by flow cytometry. All data are gated on live CD4⁺ events. *p < 0.05; **p < 0.01, Sidak's multiple comparisons test after two-way ANOVA. Bottom plots, representative IL-2 expression from splenic (D) or CNS-infiltrating (E) CD4⁺ T cells.