Table 1.
Tissue characterization, infiltration and follicle status in brain and spinal cord of progressive multiple sclerosis, Parkinson's disease, and healthy control cases.
| Case/Disease subtype | Tissue type | Number of infiltrated regions | Median score of superficial infiltration* | Median score of parenchymal infiltration* | Total median score of infiltration* | Follicle status** | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Surface | Parenchyma | Meninges | Sulcus | Cortex of brain/WM of SC | WM of brain/GM of SC | All regions | Follicle presence | Number of follicles in infiltrates | ||
| MS136/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 5 | – | – | – | 2.0 | 2.0 | + | 2/5 | |
| CALSC | 2 | 0 | 2.0 | – | – | – | 2.0 | + | 1/2 | |
| MS166/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | – | – | – | – | – | – | 0/0 | ||
| MS169/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| MS179/SPMS | CAL | 4 | 1 | 2.5 | 2.5 | – | 3.0 | 3.0 | + | 2/5 |
| MS180/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 5 | 1 | – | 3.0 | 2.0 | – | 2.5 | + | 3/6 | |
| CALSC | 6 | 2 | 3.0 | 3.0 | – | 2.0 | 2.5 | + | 4/8 | |
| MS186/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 2 | – | – | 2.0 | 2.0 | 2.0 | – | 0/2 | |
| CALSC | 1 | 1 | 1.0 | – | – | 1.0 | 1.0 | – | 0/2 | |
| MS200/SPMS | CAL | 0 | 2 | – | – | 2.0 | 1.0 | 1.5 | – | 0/2 |
| MS201/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| CALSC | 3 | 1 | 2.0 | 1.0 | 1.0 | – | 1.0 | 0/4 | ||
| MS202/SPMS | CAL | 2 | 4 | 1.5 | – | 3.0 | 2.0 | 2.0 | + | 2/6 |
| CLSC | 4 | 2 | 1.5 | – | – | 1.0 | 1.0 | + | 1/6 | |
| MS207/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 3 | 1 | – | 1.0 | – | 2.0 | 1.5 | + | 1/4 | |
| MS211/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 2 | – | – | – | 2.0 | 2.0 | + | 1/2 | |
| MS293/SPMS | CAL | 0 | 1 | – | – | – | 1.0 | 1.0 | – | 0/1 |
| CALSC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS313/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CALSC | 1 | 2 | – | 1.0 | – | 1.5 | 1.0 | – | 0/3 | |
| MS325/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 1 | – | – | – | 2.0 | 2.0 | – | 0/1 | |
| CALSC | 0 | 2 | – | – | 2.0 | 1.0 | 1.5 | – | 0/2 | |
| MS330/SPMS | CAL | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CALSC | 3 | 3 | 2.0 | 1.5 | – | 1.0 | 1.0 | – | 0/6 | |
| MS338/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 2 | 1 | – | 3.0 | 2.0 | – | 3.0 | + | 2/3 | |
| CALSC | 1 | 4 | – | 2.0 | – | 1.0 | 1.0 | + | 1/5 | |
| MS340/SPMS | NAWM | 1 | 0 | – | – | 1.0 | – | 1.0 | – | 0/1 |
| CAL | 0 | 6 | – | – | – | 1.0 | 1.0 | – | 0/6 | |
| MS363/PPMS | CAL | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CALSC | 1 | 1 | – | 1.0 | – | 1.0 | 1.0 | – | 0/2 | |
| MS383/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CALSC | 0 | 1 | – | – | – | 1.0 | 1.0 | – | 0/1 | |
| MS386/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS389/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 2 | 17 | 2.0 | 1.0 | 2.5 | 2.0 | 2.0 | + | 4/19 | |
| CALSC | 9 | 7 | 3.0 | 3.0 | – | 3.0 | 3.0 | + | 9/16 | |
| MS408/SPMS | CAL | 1 | 1 | – | 3.0 | 1.0 | – | 2.0 | + | 1/2 |
| CALSC | 2 | 0 | 1.5 | – | – | – | 1.5 | – | 0/2 | |
| MS473/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS478/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS485/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CALSC | 1 | 0 | 1.0 | – | – | – | 1.0 | – | 0/1 | |
| MS489/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS492/PPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| MS494/ | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| CAL | 0 | 2 | – | – | 2.0 | 1.0 | 1.5 | – | 0/2 | |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS497/SPMS | CAL | 0 | 8 | – | – | 2.5 | 2.0 | 2.0 | + | 2/8 |
| MS500/PPMS | CAL | 2 | 4 | 2.5 | – | – | 2.5 | 2.5 | – | 0/6 |
| MS503/SPMS | NAWM | 0 | 1 | – | – | – | 1.0 | 1.0 | – | 0/1 |
| CLSC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| MS504/SPMS | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| MS513/SPMS | CAL | 1 | 7 | – | 3.0 | 2.0 | 2.0 | 2.0 | + | 2/8 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C022 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| C025 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C032 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C036 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C037 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C039 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C045 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C048 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C052 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C054 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C059 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| C064 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| PD032 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | – | 0/0 | |
| PD034 | NAWM | 0 | 0 | – | – | – | – | – | – | 0/0 |
| NASC | 0 | 0 | – | – | – | – | – | 0/0 | ||
*
Infiltration score 1, <30 T and/or B cells, but at least five lymphocytes; score 2, 31–60 cells; score 3, >60 mean infiltrated T and B cells per region.
**
+, region with massive infiltration (score 3) plus positive staining of CD3, CD20, CD35, Ki67, and CD138; –, no or not all criteria are fulfilled.
CAL, chronic active lesional brain; CALSC, chronic active lesional spinal cord; NAWM, normal appearing white matter; NASC, normal appearing spinal cord; WM, white matter; GM, gray matter; SPMS, secondary progressive multiple sclerosis; PPMS, primary progressive multiple sclerosis; PD, Parkinson's disease; C, control cases.