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. 2020 Jan 15;10:3006. doi: 10.3389/fmicb.2019.03006

FIGURE 1.

FIGURE 1

Time course assay analyzing monomicrobial and dual species biofilms in vitro. Bacteria were grown in monomicrobial (dashed) or dual species (solid) biofilms and maintained with media changes (black arrows) for 48 h. (A) NTHi86-028 NP (blue) and M. catarrhalis 7169 (green) monomicrobial and dual species biofilms. (B) NTHi86-028 NP (blue) and S. pneumoniae EF3030 (red) monomicrobial and dual species biofilms. (C) M. catarrhalis 7169 (green) and S. pneumoniae EF3030 (red) monomicrobial and dual species biofilms. Black dashed line indicates the limit of CFU detection. Each symbol and error bar represent the mean CFU per mL and standard deviation (SD) of biofilms at the respective time point. Data presented are from three independent assays with a minimum of two biologic replicates per assay.