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Initial reaction rates of DdlA affected by concentration of ATP and D-Ala. The kinetics parameters of DdlA were calculated via the Michaelis-Menten model. The reaction rates affected by ATP and D-Ala (which were twofold diluted from 1 mM to 31.25 μM) were detected to calculate the kinetic parameters via non-linear regression of the Michaelis-Menten model in GraphPad Prism 5.0 (n = 3).
