FIGURE 5.

DdlA is essential for Mtb growth and IMB-0283 inhibits Mtb growth in a DdlA-dependent manner. A conditional mutant strain, Mtb-KD, and its sensitivities to DCS and IMB-0283 were confirmed by culturing with different concentrations of pristinamycin. (A) Wild-type and mutant Mtb H37Rv were lineated on 7H10 solid medium in the presence (+) or absence (−) of 0.1 μg/mL of pristinamycin; the former formed lawns on the medium independent of the presence of the inducer, while the latter formed colonies only on the medium containing inducer. (B) DCS inhibited mutant Mtb at 1 μg/mL or 32 μg/mL when cultured with 0.1 × 10^{– 4} μg/mL (repressed) or 0.1 μg/mL (induced) of inducer. At the same conditions, IMB-0283 inhibited the mutant strain at either 0.25 or 2 μg/mL.