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. 2020 Jan 15;10:3016. doi: 10.3389/fmicb.2019.03016

FIGURE 2.

FIGURE 2

Antibacterial effect of ebselen on Staphylococcus aureus through targeting bacterial TrxR. S. aureus LT-1 cells grown to DO 600 nm of 0.4 and diluted 100 times were treated with serial dilution of ebselen, and gentamycin was used as positive control. (A) Antibacterial effect of ebselen on the growth of S. aureus. Bacterial growth was presented by measuring OD600 nm. S. aureus LT-1 cells grown to DO 600 nm of 0.4 and were treated with 22 μg/ml ebselen. (B) Mean ± SD of propidium iodide (PI)-stained S. aureus LT-1 by Flow cytometry; (C–H) Transmission electron microscopy of S. aureus treated with ebselen; (C,D) control; (E,F) 22 μg/ml ebselen; (G,H) 64 μg/ml gentamycin; (C,E,G) 15000x; (D,F,H) 25000x; (I) TrxR activity was assayed for DTNB reduction in the presence of Trx in S. aureus LT-1 extracts; (J) Mean fluorescent intensity (MFI) Means ±SD of H2DCF-DA-stained S. aureus LT-1 were detected to present ROS level. (∗∗P < 0.01; ∗∗∗P < 0.001; student’s t-test. Data are presented as means ±SD of three independent experiments).