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. 2020 Jan 15;10:3122. doi: 10.3389/fimmu.2019.03122

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Copyright © 2020 Machado, Girola, Maia, Bergami-Santos, Morais, Azevedo, Figueiredo, Barbuto and Travassos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry of increased granzyme B secretion around lung metastatic nodules in Rb9-treated mice. (A,B) Panels show lung tissue including B16F10-Nex2 metastatic nodules in Rb9-treated mice. Immunohistochemistry staining with 3,3-diaminobenzidine for granzyme B (A) and CD4+ T cells (B). Counterstaining: hematoxylin, in blue. Magnification: x400. (C,D) Graphs represent individual nodule areas, means and ± SEM of optical densities (OD) calculated after color deconvolution on ImageJ software for at least 8 nodules on each experimental group. ^**p < 0.01 Mann–Whitney t-test as compared to Veh.