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. 2020 Jan 15;10:3122. doi: 10.3389/fimmu.2019.03122

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Copyright © 2020 Machado, Girola, Maia, Bergami-Santos, Morais, Azevedo, Figueiredo, Barbuto and Travassos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rb9 interacts with and is internalized by bone marrow derived dendritic cells (BMDCs). Panels are representative confocal images of bmDCs stained with DAPI (blue, panel 1) and phalloidin (red, panel 3) for nucleic acid and filamentous actin staining, respectively. Biotinyl-Rb9 is stained with FITC (green, panel 2). A merge of 1, 2, and 3 can be seen on panel 4. FITC-biotinyl-Rb9 colocalizes with DAPI in a nuclear region (panel 5) and peripherally with phalloidin (panel 6) as shown by white and yellow points/areas in the cellular cytosol on both colocalization panels. Bars = 10 μm.