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. 2020 Jan 15;10:3122. doi: 10.3389/fimmu.2019.03122

Figure 5.

Figure 5

Specific binding of Rb9 peptide to MIF or CD74 recombinant proteins. (A) Dot-blotting showing specific interaction of Rb9 peptide with rCD74 and rMIF proteins. No reaction was seen with Rb10A1 peptide or the peptide vehicle (Veh); (B) Rb9 increases the expression of CD74 in CD11b+CD11c+ iDCs; (C) Increased M1 MΦ marker expression (CD86+ and MHC-II+) in bmMΦs incubated with Rb9 in the presence of melanoma factors (B16F10 conditioned medium, B16.CM); (D) Decreased M2 MΦ marker expression (PD-L1+ and CD206+) in bmMΦs incubated with Rb9 in the presence of melanoma factors (B16.CM).