Figure 2.

The relative transcript levels of TaRPM1 in different wheat tissues and in response to different hormones. (A) Tissue-specific expression level of TaRPM1. (B) Responses to hormones: ABA, abscisic acid; ET, ethylene; SA, salicylic acid; MeJA, methyl jasmonate. The mock control was treated with 0.1% (v/v) ethanol. Wheat leaves were sampled at 0, 0.5, 2, 6, 12, and 24 h post–hormone treatment. There were three biological replicates for each treatment and each sampling time point. Three technical replicates for each sample were conducted. Relative transcript levels of TaRPM1 were calculated using the comparative threshold (2^−ΔΔCT) method, relative to the mock control at every sampling point. The expression level was standardized as 1 at 0 h. Duncan’s multiple comparison test was conducted to compare between time points for each hormone treatment. The TaRPM1 expression levels do not differ significantly if they contain at least one common lowercase letter among time points for each hormone treatment.