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. 2020 Jan 15;10:1679. doi: 10.3389/fpls.2019.01679

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Copyright © 2020 Wang, Tian, Tao, Wang, Shang, Chen, Xu and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological observations of Pst development and host responses in TaRPM1-silenced leaves under normal temperature. The images of the fourth leaves inoculated with BSMV:00 (A, D), BSMV: TaRPM1-1as (B, E), and BSMV: TaRPM1-2as (C, F) were taken under a ﬂuorescence microscope at 48 hpi (A, B, C, bar, 50 μm) and 120 hpi (D, E, F, bar, 100 μm). SV, substomatal vesicle; HMC, haustorial mother cell; IH, infection hypha; SH, secondary hypha. Hyphal length (G) and the number of haustorial mother cells (H) were assessed at 48 and 120 hpi using the DP-BSW software after Pst were stained. There are no significant differences at the same time point among treatments if they share at least one common lowercase letter. Three independent biological replicates were performed for each treatment and sampling time combination.