Figure 1.
Localization analysis of GFP fused to the mutant transit peptide of PARC6 (TPG62R-GFP). GFP with or without a PARC6 transit peptide (TP) sequence from Col or parc6-5 (TPG62R) was transiently expressed in tobacco and onion cells by particle bombardment. (A–F) Epifluorescence microscopy images of tobacco leaf pavement cells and guard cells and onion epidermal cells at 24 h (A–C) or 6 h (D–F) post-bombardment. GFP fluorescence, chlorophyll autofluorescence (Chl), and merged images are shown. Scale bars: 20 µm (all guard cells and insets); 50 µm (others).