Figure 5.

Distribution of chloroplasts in leaf stomatal guard cells of Arabidopsis parc6 and other plastid division mutants. (A, B) Measurements of chloroplast number in arbitrarily selected 100 guard cell (described as “GC” in this figure) pairs (“cell A” and “cell B”) of WT, parc6-1, parc6-3, parc6-4, parc6-5 and arc11-1 seedlings in graph (A) and table (B) form. All plants were derived from the FL4-4 line, and the 3^rd and 4^th leaf petioles of 4-week-old seedlings were examined. (C) Frequency of guard cells with giant, normal-sized, or no chloroplasts in parc6-1, arc11-1, and other mutant plants, based on results in the present study and previous study (Fujiwara et al., 2018). The fluorescence assays were representative of several measurements. Three to five independent leaves were used for each measurement.