TABLE 2.

Divergence of markers in the four species of Hanseniaspora.

	H. uvarum					H. guilliermondii					H. opuntiae					H. pseudoguilliermondii
MLST locus	ADP1	GLN4	MET5	RPN2	VPS13	ADP1	GLN4	MET5	RPN2	VPS13	ADP1	GLN4	MET5	RPN2	VPS13	ADP1	GLN4	MET5	RPN2	VPS13
# Sequences	50	50	50	50	50	24	24	24	24	24	26	26	26	26	26	9	9	9	9	9
# total sites	445	559	909	951	566	440	556	917	973	563	419	564	859	961	551	432	556	918	953	561
# heterozygous sites1	10	16	54	50	21	0	2	0	5	0	21	8	80	59	33	4	5	0	1	2
# polymorphic sites2	17	21	59	60	24	7	4	21	11	5	22	8	88	60	35	5	5	16	14	5
% polymorphic sites	3.82	3.76	6.49	6.31	4.24	1.59	0.72	2.29	1.13	0.89	5.25	1.42	10.24	6.24	6.35	1.15	0.90	1.74	1.47	0.89
Nucleotide diversity (Π)3	4.35	2.87	14.52	17.33	10.00	2.52	1.79	11.12	6.53	3.37	4.62	2.21	40.55	16.9	16.76	3.39	4.11	9.89	10.89	3.33

¹The number of heterozygous sites refers to the number of positions where at least one strain has a heterozygous position. ²The number of polymorphic sites refers to the number of positions where at least two alleles are present in the strain population, including heterozygous positions. ³Nucleotide diversity is measured by the average number of nucleotide differences between all pairs of strains in each alignment.