FIG 3.

Intracellular colocalization of mGBP proteins at the PVM of T. gondii. Recruitment and colocalization of mGBPs was analyzed in G-mGBP7/mCh-mGBP (1, 2, 3, 5, 6, and 7) MEFs. mCherry-expressing cells served as controls. Cells were stimulated with IFN-γ for 16 h and subsequently infected with T. gondii for 2 h. After fixation, T. gondii was detected with an α-SAG1 antibody, and nuclei of cells were stained with DAPI. Glass slides were analyzed by confocal microscopy. Bars, 5 μm. Colocalization analysis was computed with Imaris (Bitplane).