TABLE 2.

Cryo-EM model statistics

Model statistica	Value for the structure
	E site occupied	P site occupied	Empty	50S	30S
Refinement
Resolution	2.82	3.04	2.91	2.95	4.4
FSC threshold	0.143	0.143	0.143	0.143	0.143
Map resolution range (Å)	2.39–30	2.32–30	2.35–30	2.62–30	2.26–30
Model cutoff (Å)	3	3.1	3	3	4.5
Model composition
Nucleotides	4,615	4,615	4,535	3,007	1,528
Protein residues	5,457	5,458	5,457	3,117	2,311
RMSD
Bond lengths (Å)	0.002	0.002	0.002	0.002	0.002
Bond angles (°)	0.484	0.460	0.479	0.448	0.434
Validation
MolProbity score	1.48	1.45	1.49	1.48	1.65
Clash score	5.95	6.16	5.91	5.91	11.21
Poor rotamers (%)	0.07	0.00	0.05	0.04	0.05
Ramachandran plot (%)
Favored	97.16	97.43	97.05	97.09	97.62
Allowed	2.76	2.52	2.89	2.81	2.38
Disallowed	0.07	0.06	0.06	0.1	0
CCmask	0.82	0.85	0.83	0.86	0.74
CCbox	0.82	0.86	0.83	0.85	0.88
CCvol	0.81	0.83	0.82	0.85	0.74

^aCC, correlation coefficient.