Effect of omega-3 polyunsaturated fatty acid (PUFA) supplementation on urinary gonadotropins. Daily morning urine was assayed for luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and normalized to creatinine. Cycles were aligned based on the Day of Luteal Transition (Day 0). Data are from normal weight (NW) women (blue; n = 7) and obese women (red; n = 10). For each hormone, NW subjects are compared at baseline (open circles, solid line) and after omega-3 supplementation (closed circles, dashed line; panel A for LH; panel D for FSH). Obese subjects are compared at baseline (open circles, solid line) and after omega-3 supplementation (closed circles, dashed line; panel B for LH; panel E for FSH). Error bars indicate standard error of the mean for group composites. Panels C and F represent data for area under the curve (AUC) at baseline (solid bars) and post omega-3 supplementation (hashed bars) by menstrual cycle phase. All significant differences between LH and FSH measurements are indicted by asterisk, as follows. All other comparisons performed on the LH and FSH measurements were not significant *P = .02. **P = .04. ***P = .01. Data are medians; error bars indicate interquartile range (IQR).