Figure 5.

Ex vivo studies on isolated bone marrow neutrophils of Wilson (tx-J) and Menkes (^ms/−) disease mice and their respective controls (ctr-tx and ctr-7a) evaluating their capacity to form NETs (A), adhere (B), and release reactive oxygen species (ROS; C). NET formation and capacity to adhere were evaluated 6 h after stimulation with LPS, and the content of ROS was measured after the first hour (DCF-DA test). In (C), white/black bars represent responses of cells treated with H₂O₂ (positive control)—data for neutrophils from control mice (white bars) and mutants (black bars). Asterisks indicate significant differences using unpaired two-tailed Student's t test (*P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001) and one-way ANOVA (post hoc Bonferroni) test (different letters indicate statistically significant differences between groups).