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The impact of subchronic copper deficiency on NET formation (A), neutrophil numbers (B), Kupffer cell counts (C), and platelet covered area (D) in C57Bl/6J mice. Copper chelator (Cu chelator) was administered for 8 days and then LPS-systemic inflammation was induced. NET covered area and cell counts were performed 24 h later. Asterisks indicate significant differences using unpaired two-tailed Student's t test (***P ≤ 0.001, ****P ≤ 0.0001).
