Figure 8.

The impact of exogenous copper of neutrophil viability (A), and counteraction of copper effects by its chelating agent (B) in ex vivo conditions. Neutrophils isolated from the bone marrow of C57Bl/6J mice were incubated with various copper concentrations in the presence (+LPS) or absence (–LPS) of lipopolysaccharide (A). Cell viability reversed by the Cu chelator (B). The dotted line (B) indicates values for untreated neutrophils (Cu concentration “0” in A). Asterisks indicate significant differences using unpaired two-tailed Student's t test (**P ≤ 0.01) and one-way ANOVA (post hoc Bonferroni) test (different letters indicate statistically significant differences between groups).