FIG 6.

Activation of duramycin biosynthesis. Overlay bioassays were carried out with B. subtilis EC1524, and agar plugs were taken adjacent to the central streak and analyzed by UPLC-MS. Extracted ion chromatograms are shown for m/z 1,006.93 ([M + 2H]²⁺). Duramycin was only detected in the strain carrying both pOJKKH and pEVK6. The duramycin peak aligns with an authentic standard of duramycin (0.1 mg/ml in 5% formic acid), shown on a separate scale. Images and LC traces are representative of at least three biological repeats.