TABLE 3.
Strains and plasmids used in this work
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Saccharopolyspora sp. | ||
| KY3 | Strain from the cuticles of Tetraponera penzigi | This work |
| KY3/pGP9 | KY3 strain carrying the empty pGP9 plasmid | This work |
| KY3/pEVK4 | KY3 strain carrying pEVK4, which activates kyamicin production | This work |
| KY7 | Strain from the cuticles of Tetraponera penzigi | This work |
| KY7/pGP9 | KY7 strain carrying the empty pGP9 plasmid | This work |
| KY7/pEVK4 | KY7 strain carrying pEVK4, which activates kyamicin production | This work |
| KY21 | Strain from the cuticles of Tetraponera penzigi | This work |
| KY21/pGP9 | KY21 strain carrying the empty pGP9 plasmid | This work |
| KY21/pEVK4 | KY21 strain carrying pEVK4, which activates kyamicin production | This work |
| Streptomyces coelicolor | Non-antibiotic-producing superhost, Δact Δred Δcpk Δcda rpoB(C1298T) | 43 |
| M1152/pEVK6 | M1152 carrying pEVK6 | This work |
| M1152/pWDW63 | M1152 carrying the kyamicin biosynthetic genes | This work |
| M1152/pEVK6/pWDW63 | M1152 carrying the kyamicin biosynthetic genes and pEVK6, which activates kyamicin production | This work |
| M1152/pWDW63/pEVK12 | M1152 carrying the kyamicin biosynthetic genes pEVK12 for constitutive expression of kyaL | This work |
| M1152/pWDW63/pEVK13 | M1152 carrying the kyamicin biosynthetic genes pEVK13 for constitutive expression of kyaR1 | This work |
| M1152/pOJKKH | M1152 carrying the duramycin biosynthetic genes | This work |
| M1152/pEVK6/pOJKKH | M1152 carrying the duramycin biosynthetic genes and pEVK6, which activates duramycin production | This work |
| Bacillus subtilis EC1524 | Bioassay strain; trpC2, subtilin BGC deleted | 26 |
| Plasmids | ||
| pGP9 | pSET152-derived φBT-based integrative expression vector | 42 |
| pIJ10257 | oriT, φBT1 attB-int, Hygr, ermEp* | 44 |
| pSET152 | ϕC31 attP-conjugative vector | 45 |
| pEVK1 | pUC57/R1L Synthetic construct with kyaR1L genes | GenScript |
| pEVK4 | pGP9/R1L for constitutive expression of kyaR1L in Saccharopolyspora | This work |
| pEVK6 | pIJ10257/R1L for constitutive expression of kyaR1L in S. coelicolor | This work |
| pEVK12 | pIJ10257/L for constitutive expression of kyaL in S. coelicolor | This work |
| pEVK13 | pIJ10257/R1 for constitutive expression of kyaR1 in S. coelicolor | This work |
| pWDW60 | pUC57/Kya synthetic construct with the kyamicin biosynthetic genes kyaN to kyaH | GenScript |
| pWDW63 | pSET152/Kya for constitutive expression of the kyamicin biosynthetic genes in S. coelicolor | This work |
| pOJKKH | pOJ436-based plasmid carrying the duramycin biosynthetic genes durN to durZ | This work |