Strains |
|
|
Saccharopolyspora sp. |
|
|
KY3 |
Strain from the cuticles of Tetraponera penzigi
|
This work |
KY3/pGP9 |
KY3 strain carrying the empty pGP9 plasmid |
This work |
KY3/pEVK4 |
KY3 strain carrying pEVK4, which activates kyamicin production |
This work |
KY7 |
Strain from the cuticles of Tetraponera penzigi
|
This work |
KY7/pGP9 |
KY7 strain carrying the empty pGP9 plasmid |
This work |
KY7/pEVK4 |
KY7 strain carrying pEVK4, which activates kyamicin production |
This work |
KY21 |
Strain from the cuticles of Tetraponera penzigi
|
This work |
KY21/pGP9 |
KY21 strain carrying the empty pGP9 plasmid |
This work |
KY21/pEVK4 |
KY21 strain carrying pEVK4, which activates kyamicin production |
This work |
Streptomyces coelicolor
|
Non-antibiotic-producing superhost, Δact Δred Δcpk Δcda rpoB(C1298T) |
43 |
M1152/pEVK6 |
M1152 carrying pEVK6 |
This work |
M1152/pWDW63 |
M1152 carrying the kyamicin biosynthetic genes |
This work |
M1152/pEVK6/pWDW63 |
M1152 carrying the kyamicin biosynthetic genes and pEVK6, which activates kyamicin production |
This work |
M1152/pWDW63/pEVK12 |
M1152 carrying the kyamicin biosynthetic genes pEVK12 for constitutive expression of kyaL
|
This work |
M1152/pWDW63/pEVK13 |
M1152 carrying the kyamicin biosynthetic genes pEVK13 for constitutive expression of kyaR1
|
This work |
M1152/pOJKKH |
M1152 carrying the duramycin biosynthetic genes |
This work |
M1152/pEVK6/pOJKKH |
M1152 carrying the duramycin biosynthetic genes and pEVK6, which activates duramycin production |
This work |
Bacillus subtilis EC1524 |
Bioassay strain; trpC2, subtilin BGC deleted |
26 |
Plasmids |
|
|
pGP9 |
pSET152-derived φBT-based integrative expression vector |
42 |
pIJ10257 |
oriT, φBT1 attB-int, Hygr, ermEp* |
44 |
pSET152 |
ϕC31 attP-conjugative vector |
45 |
pEVK1 |
pUC57/R1L Synthetic construct with kyaR1L genes |
GenScript |
pEVK4 |
pGP9/R1L for constitutive expression of kyaR1L in Saccharopolyspora
|
This work |
pEVK6 |
pIJ10257/R1L for constitutive expression of kyaR1L in S. coelicolor
|
This work |
pEVK12 |
pIJ10257/L for constitutive expression of kyaL in S. coelicolor
|
This work |
pEVK13 |
pIJ10257/R1 for constitutive expression of kyaR1 in S. coelicolor
|
This work |
pWDW60 |
pUC57/Kya synthetic construct with the kyamicin biosynthetic genes kyaN to kyaH
|
GenScript |
pWDW63 |
pSET152/Kya for constitutive expression of the kyamicin biosynthetic genes in S. coelicolor
|
This work |
pOJKKH |
pOJ436-based plasmid carrying the duramycin biosynthetic genes durN to durZ
|
This work |