TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description (isolation location) | Reference or source |
|---|---|---|
| Strains | ||
| L. brevis | ||
| UCCLBBS124 (UCC124) | Beer-spoiling strain isolated from spoiled beer keg (Singapore) | 10 |
| UCCLBBS449 | Beer-spoiling strain isolated from unpasteurized spoiled beer (The Netherlands) | 10 |
| MB569 | Non-beer-spoiling strain derivative of UCCLBBS124 | This study |
| UCCLB521 (UCC521) | Non-beer-spoiling strain isolated from a brewery environment (The Netherlands) | 10 |
| MB569/pNZ44 | MB569 carrying pNZ44 | This study |
| MB569/pNZ44:gtfD15 | MB569 carrying pNZ44 with gtfD15 | This study |
| UCCLB521/pNZ44:gtfD15 | UCCLB521 carrying pNZ44 with gtfD15 | This study |
| L. lactis | ||
| NZ9000 | Transformation host | 34 |
| Plasmids | ||
| pNZ44 | Transformation vector, chloramphenicol resistance gene | |
| pNZ44:gtfD15 | pNZ44 harboring gtfD15 | This study |