FIG 4.

Transcript abundance for predicted chain elongation transcripts by “Ca. Weimeria bifida.” “Prefed” represents the time point prior to feeding the reactor. Error bars show 95% confidence levels determined with CuffLinks. Transcript abundance data are shown for transcripts encoding enzymes used for reverse β-oxidation (A), the RNF complex (B), hydrogenases (C), and terminal enzymes of reverse β-oxidation (D). The roles of the designated enzymes in chain elongation are provided in Fig. 2. FPKM, fragments per kilobase per million.