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. 2020 Jan 21;86(3):e02334-19. doi: 10.1128/AEM.02334-19

TABLE 1.

Results from comparative statistical analyses for measures of alpha and beta diversity and SCML read counts across groups within each independent data seta

Data set and metric DF Numb DF Denb Fb Pb CV (%) Asymptotic test
MSLRT
Value CV P Value CV P
Treatment Nonfungal Fungal
Larva type
    Simpson 1 5.947 0.798 0.406 9.02 5.91 3.119 0.077 3.377 0.066
    Shannon 1 5.925 1.941 0.214 17.65 11.77 2.783 0.095 2.874 0.090
    Bray-Curtis 1 (1) 6 (36) 1.042 (0.537) 0.442 (0.489)
    Unweighted UniFrac 1 (1) 6 (36) 1.085 (4.653) 0.398 (0.036)
    Weighted UniFrac 1 (1) 6 (36) 0.977 (0.297) 0.424 (0.597)
    Jaccard 1 (1) 6 (36) 0.959 (1.116) 0.791 (0.323)
Adult type
    Simpson 1 5.975 4.039 0.091 39.64 8.42 33.292 <0.001 37.175 <0.001
    Shannon 1 5.978 3.277 0.120 54.45 17.76 18.023 <0.001 19.251 <0.001
    Bray-Curtis 1 (1) 6 (31) 1.700 (2.711) 0.088 (0.1)
    Unweighted UniFrac 1 (1) 6 (31) 1.493 (0.860) 0.084 (0.383)
    Weighted UniFrac 1 (1) 6 (31) 5.180 (2.701) 0.06 (0.128)
    Jaccard 1 (1) 6 (31) 1.427 (0.939) 0.075 (0.356)
Developmental stage Larval Adult
Nonfungal mosquitoes
    Simpson 1 5.993 1.468 0.271 9.02 39.64 31.177 <0.001 33.340 <0.001
    Shannon 1 5.995 0.006 0.939 17.65 54.45 18.189 <0.001 34.281 <0.001
    Bray-Curtis 1 (1) 6 (34) 3.705 (31.461) 0.022 (<0.001)
    Unweighted UniFrac 1 (1) 6 (34) 6.968 (43.821) 0.035 (<0.001)
    Weighted UniFrac 1 (1) 6 (34) 8.919 (0.052) 0.036 (0.804)
    Jaccard 1 (1) 6 (34) 2.512 (23.523) 0.037 (<0.001)
    SCML 1 6 10.183 0.019
Fungal mosquitoes
    Simpson 1 5.510 3.227 0.127 5.91 8.42 2.232 0.135 2.279 0.131
    Shannon 1 5.510 13.903 0.011 11.77 17.76 2.860 0.091 2.960 0.085
    Bray-Curtis 1 (1) 6 (29) 6.299 (32.067) 0.028 (<0.001)
    Unweighted UniFrac 1 (1) 6 (29) 10.743 (53.22) 0.03 (<0.001)
    Weighted UniFrac 1 (1) 6 (29) 8.859 (0.061) 0.025 (0.79)
    Jaccard 1 (1) 6 (29) 3.737 (32.723) 0.029 (<0.001)
    SCML 1 6 6.974 0.038
a

Significant differences of mean alpha diversity measures and SCML calibrated read counts were calculated with linear mixed models, CV values were calculated with asymptotic and modified signed-likelihood ratio tests (MSLRT), beta diversity measures were calculated with nested permutational multivariate analysis of variance, and within-group variations of beta diversity measures were calculated with permutational statistical tests for the homogeneity of group dispersions. Values in boldface are significant (P < 0.05).

b

Values in parentheses represent results from homogeneity of group dispersion tests. DF Den, denominator degrees of freedom; DF Num, numerator degrees of freedom.