TABLE 2.
Primer sequences used in experimental PCRs
| Target and primer | Sequence |
||||
|---|---|---|---|---|---|
| Adapter | Spacer | Linker | Primer | Final | |
| 16S rRNA gene | |||||
| 341f_1 | ACACTGACGACATGGTTCTACA | AG | CCTACGGGNGGCWGCAG | ACACTGACGACATGGTTCTACAAGCCTACGGGNGGCWGCAG | |
| 341f_2 | ACACTGACGACATGGTTCTACA | T | AG | CCTACGGGNGGCWGCAG | ACACTGACGACATGGTTCTACATAGCCTACGGGNGGCWGCAG |
| 341f_3 | ACACTGACGACATGGTTCTACA | CT | AG | CCTACGGGNGGCWGCAG | ACACTGACGACATGGTTCTACACTAGCCTACGGGNGGCWGCAG |
| 341f_4 | ACACTGACGACATGGTTCTACA | GCT | AG | CCTACGGGNGGCWGCAG | ACACTGACGACATGGTTCTACAGCTAGCCTACGGGNGGCWGCAG |
| 785r_1 | TACGGTAGCAGAGACTTGGTCT | CT | GACTACHVGGGTATCTAATCC | TACGGTAGCAGAGACTTGGTCTCTGACTACHVGGGTATCTAATCC | |
| 785r_2 | TACGGTAGCAGAGACTTGGTCT | T | CT | GACTACHVGGGTATCTAATCC | TACGGTAGCAGAGACTTGGTCTTCTGACTACHVGGGTATCTAATCC |
| 785r_3 | TACGGTAGCAGAGACTTGGTCT | AT | CT | GACTACHVGGGTATCTAATCC | TACGGTAGCAGAGACTTGGTCTATCTGACTACHVGGGTATCTAATCC |
| 785r_4 | TACGGTAGCAGAGACTTGGTCT | GAT | CT | GACTACHVGGGTATCTAATCC | TACGGTAGCAGAGACTTGGTCTGATCTGACTACHVGGGTATCTAATCC |
| 18S rRNA gene | |||||
| TR3F | GGCACTGTCAGTGGTGAAATAC | GGCACTGTCAGTGGTGAAATAC | |||
| TR4R | GATTTCTCTTACGGTGCCAAGCA | GATTTCTCTTACGGTGCCAAGCA | |||