TABLE 2.

PCR primers and bacterial isolates used in this work

Gene or strain	Primer name	Primer (5′ to 3′)a	Expected amplicon size (bp)	Source	Reference
Gene
cbiL	Precorrin-2 forward	TYTTCAGCATGTCSCGYGAC	358		This work
cbiL	Precorrin-2 reverse	GCGGCTRCGGTAGGTYTT	358		This work
cbiC	cbiC forward	ATCCACACCACGGCRGAC	500		This work
cbiC	cbiC reverse	GGCGTGCAGGGTRGT	500		This work
cbiFGH	cbiG forward	GTSAGCAGCGTYTTYG	340		This work
cbiFGH	cbiG reverse	ATGAGSGCCTGCCKKCCGA	340		This work
cdiD	cbiD forward	GACCCSGACTGCACSCA	379		This work
cdiD	cbiD reverse	TAGGCTTCRTGGCTG	379		This work
16S rRNA	8F	AGAGTTTGATCCTGGCTCAG	Variable		79
16S rRNA	515F	GTGCCAGCMGCCGCGGTAA	Variable		79
16S rRNA	806R	GGACTACHVGGGTWTCTAAT	Variable		80
16S rRNA	1492R	TACGGTTACCTTGTTACGA	Variable		81
Strain
A. muciniphila MucT				Human adult male; ATCC BAA-835	13
A. glycaniphila PytT				Reticulated python; DSM 100705	27
A. muciniphila CSUN-7				Human adult male	This work
A. muciniphila CSUN-12				Human adult male	This work
A. muciniphila CSUN-17				Human adult male	This work
A. muciniphila CSUN-23				Human adult male	This work
A. muciniphila CSUN-27				Human adult male	This work
A. muciniphila CSUN-28				Human adult male	This work
A. muciniphila CSUN-31				Human adult female	This work
A. muciniphila CSUN-33				Human adult male	This work
A. muciniphila CSUN-34				Human adult male	This work
A. muciniphila CSUN-36				Human adult female	This work

^aY = C or T, S = C or G, B = G, T, or C, and R = G or A.