Table 6.
Phenotypic characteristics of M. kansasii strains under the study.
| Type/Strain no.a | Morphologyb | Pigmentc | Growth in/post 7 days at/ond: | Tolerance ofe: | Niacin | Reduction of: | Arylsulfatase | Tween 80 hydrolysisf | Catalaseg | Ureaseh | PZAi | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SQ | HS | |||||||||||||||||||
| 25°C | 37°C | 45°C | MCA | TCH | NaCl 5% | nitrate | tellurite | 3–day | 14–day | |||||||||||
| Mycobacterium kansasii* | SR | P99; S <1; N <1 | + | + | – | –99 | +99 | –99 | –99 | +99 | –84 | –99 | +55 | +99 | >4599 | +95 | +97 | –99 | ||
| M. kansasii | I (11) | NLA001000521 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 17 | – | – | – |
| ATCC25221 | RW | N | + | + | – | – | + | – | – | + | – | – | 3+ | + | 74 | + | + | – | ||
| NLA001000927 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 54 | + | + | – | ||
| NLA001000449 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 50 | + | – | – | ||
| 6200 | RY | P | + | + | – | – | + | – | – | + | – | – | 4+ | + | 52 | + | – | – | ||
| 7728 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 70 | + | – | – | ||
| 7744 | RY | P | + | + | – | – | + | – | – | + | – | – | 2+ | + | 59 | + | – | – | ||
| 1010001495 | RY | P | + | + | –/+ | – | + | – | – | + | – | – | 4+ | + | 65 | + | – | – | ||
| K4 | RY | P | + | + | –/+ | – | + | – | – | + | – | – | 3+ | + | 54 | + | – | – | ||
| K14 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 41 | + | + | – | ||
| K19 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 51 | + | + | – | ||
| RY91 | P91 | +100 | +100 | –82 | –100 | +100 | –100 | –100 | +100 | –100 | –100 | ≥3+91 | +100 | >4091 | +91 | –64 | –100 | |||
| M. persicum | II (8) | 2193 | RY | P | + | + | – | – | + | – | – | – | – | – | 3+ | – | 44 | + | – | – |
| NLA001001128 | RY | P | + | + | – | – | + | – | – | + | + | – | 3+ | – | 40 | + | + | – | ||
| B11073207 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | – | 44 | + | + | – | ||
| B11063838 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | – | 43 | + | + | – | ||
| 1010001469 | RY | P | + | + | – | – | + | – | – | + | – | – | 4+ | + | 18 | – | – | – | ||
| H47 | RY | P | + | + | – | – | + | – | – | + | + | – | 4+ | + | 45 | + | + | – | ||
| H48 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 47 | + | + | – | ||
| AFPC−000227 (T)** | RY | P | – | – | – | – | NT | NT | – | + | – | NT | NT | + | >45 | + | – | NT | ||
| RY100 | P100 | +88 | +88 | –100 | –100 | +100 | –100 | –100 | +88 | –75 | –100 | ≥3+100 | +50 | ≥4088 | +88 | +63 | –100 | |||
| M. pseudokansasii | III (4) | 14_15 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 9 | – | + | – |
| 174_15 | RY | P | + | + | – | – | + | – | – | + | – | – | 1+ | + | 8 | – | + | – | ||
| 1010001468 | RY | P | + | + | – | – | + | – | – | + | + | – | 3+ | + | 30 | + | – | – | ||
| MK142 | RY | P | + | + | – | – | + | – | – | + | – | – | 2+ | + | 45 | + | + | – | ||
| MK142 (T)*** | RY | NT | NT | NT | NT | NT | NT | NT | – | + | NT | NT | NT | + | NT | NT | v | NT | ||
| RY100 | P100 | +100 | +100 | –100 | –100 | +100 | –100 | –100 | +100 | –75 | –100 | ≥2+75 | +100 | ≥3050 | +50 | +60 | –100 | |||
| M. ostraviense | IV (2) | 1010001458 | RY | P | + | + | – | – | + | – | – | – | – | – | 1+ | – | 53 | – | – | – |
| 241/15 (T) | RY | P | + | + | – | – | + | – | – | – | – | – | 1+ | + | 48 | – | – | – | ||
| RY100 | P100 | +100 | +100 | –100 | –100 | +100 | –100 | –100 | –100 | –100 | –100 | ≥1+100 | +50 | >40100 | –100 | –100 | –100 | |||
| M. innocens | V (4) | 1010001454 | RY | P | + | + | – | – | + | – | – | + | – | – | 2+ | + | 78 | + | + | – |
| 1010001493 | RW | N | + | + | – | – | + | – | – | + | – | – | 1+ | + | 3 | – | – | – | ||
| 49_11 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | + | 43 | + | + | – | ||
| MK13 (T) | RY | P | + | + | – | – | + | – | – | + | – | – | 1+ | – | 39 | + | + | – | ||
| MK13 (T)*** | Y | NT | NT | NT | NT | NT | NT | NT | – | v | NT | NT | NT | v | NT | NT | v | NT | ||
| RY60 | P80 | +100 | +100 | –100 | –100 | +100 | –100 | –100 | +80 | –100 | –100 | ≥1+100 | +60 | >3075 | +75 | +60 | –100 | |||
| M. attenuatum | VI (2) | NLA001001166 | RY | P | + | + | – | – | + | – | – | + | – | – | 3+ | – | 60 | + | + | – |
| MK41 (T) | RY | P | + | + | – | – | + | – | – | + | – | – | 2+ | + | 45 | + | + | – | ||
| MK41 (T)*** | Y | NT | NT | NT | NT | NT | NT | NT | v | v | NT | NT | NT | + | NT | NT | v | NT | ||
| RY67 | P100 | +100 | +100 | –100 | –100 | +100 | –100 | –67 | +67 | –100 | –100 | ≥2+100 | +67 | >40100 | +100 | +67 | –100 | |||
All test reactions are given as “+” (positive), “–” (negative) or “v” (variable); NT, not tested. Numbers in superscripts represent percentage of strains reacting as indicated.
According to the WGS-based (MiSI method) grouping. Vertically are given the newly proposed names for each of the M. kansasii subtype; (T), type strain.
Results according to Kent and Kubica (1985); prior to subtype delineation.
Results according to Shahraki et al. (2017).
Results according to Tagini et al. (2019).
Colony morphology: S, smooth; R, rough; Y, yellow/beige; W, white.
Photochromogenicity: N, non-photochromogenic; P, photochromogenic; S, scotochromogenic.
MCA, MacConkey agar without crystal violet.
TCH, thiophene-2-carboxylic acid hydrazide.
After 10 days.
SQ, semi-quantitative catalase [mm]; HS, heat-stable catalase.
after 5 days; v, variable.
PZA, pyrazinamidase activity, after 4 days.