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. 2019 Oct 17;19(1):e13051. doi: 10.1111/acel.13051

Figure 3.

Figure 3

ATXN3 acts upstream of p97/VCP in the autophagy pathway. (a) Knockdown efficiencies of siRNA oligos targeting ATXN3 and/or p97/VCP after 48 hr. (b) Representative micrographs of HOS GFP‐LC3 cells depleted of ATXN3, p97/VCP, or both using the indicated siRNAs. Cells were starved in the presence of 100 nM bafilomycin A1 for 4 hr. Scale bar: 20 μm. (c) Quantification of GFP‐LC3 puncta in (b). Data are shown as box plot with median and 5–95 percentile of two independent experiments, n > 1,200, *p ≤ .05, **p ≤ .01, ***p ≤ .001 (Kruskal–Wallis test)