Figure 5.

Levels of mRNA expression of the main inflammatory mediators in young, RS, and EIS fibroblasts of Spalax, human, and mice. The mRNA expression rates were quantified by using qRT–PCR. Representative data for IL1α, IL6, SerpinB2, GROα, and ICAM‐1 are shown. Additional independent biological repeats [cells isolated from different animals (Spalax and mice) or different experiments (human cells)] are presented in Figures S2a, S3, and S4. Treatment of Spalax fibroblasts with MDA‐MB‐231 CM was used for the induction of pro‐inflammatory cytokines as a positive control. The top right panel demonstrates the increase in IL10 mRNA in Spalax senescent cells. Bars represent mean ± SD of three independent biological experiments (n = 3; * p ≤ .05). Note: The IL10 mRNA expression was undetectable in human and mouse cells. Etop, etoposide; RS, replicative senescence