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. 2020 Jan 14;30:101433. doi: 10.1016/j.redox.2020.101433

Fig. 1.

Fig. 1

IS impairs NO generation and attenuates valsartan-mediated NO production and tube formation in HAECs. (A) HAECs were treated with IS (0, 15, 30, 60, 120, 240 μg/mL) for 18 h. (B) HAECs were treated with 240 μg/mL IS for the indicated times (0, 3, 6, 9, 12, 18 h). The level of nitrite in the culture medium was measured by the Griess assay. (C) HAECs were pretreated with IS (15, 30, 60, 120, 240 μg/mL) for 1 h and then with valsartan (10 μM) for 18 h. (D) HAEC proliferation or (E) migration was determined by BrdU incorporation assay or wound healing migration assay, respectively. (F) HAECs were cultured in precoated ECL Cell Attachment Matrix with the indicated treatment agents. Tube formation was visualized; the bar graphs indicate the fold of branch points in 5 randomly selected microscopy views. (G–J) HAECs were pretreated with indicated concentrations of IS for 1 h in the presence of human serum albumin (4  g/dL) and then with valsartan (10 μM) for 18 h. (G) Nitrite level in the culture medium, (H) proliferation, (I) migration, and (J) tube formation were determined. Values are presented as the mean ± SEM of 5 separate experiments. *P < 0.05 vs. vehicle-treated cells, #P < 0.05 vs. valsartan-treated cells.