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. 2020 Jan 14;30:101433. doi: 10.1016/j.redox.2020.101433

Fig. 2.

Fig. 2

Effects of IS on eNOS phosphorylation at various sites in HAECs. HAECs were treated with IS (240 μg/mL) for the indicated times (0, 0.5, 1, 2, 4, 8, 12, 18 h). (A–E) Cellular lysates were subjected to Western blot analysis to evaluate the phosphorylation of eNOS at Thr495, Ser615, Ser633, Ser1177 or eNOS. Data are expressed as the mean ± SEM. *P < 0.05 vs. vehicle-treated cells.