Figure 5.

SLC7A11 expression predicts and affects ESCC sensitivity to oridonin. (A) Transcriptional expression of the SLC7A11 gene in esophageal squamous carcinoma cells. (B–D) KYSE70, KYSE410. KYSE30 and KYSE450 cells were treated with different concentrations of oridonin or vehicle for 24 h (B), 48 h (C), and 72 h (D). The cell's inhibition rate was determined using MTT assay, (E) IC50 values comparison for them. (F) Oridonin was applied 72 h post-transfection of SLC7A11 and non-targeting control (NC) siRNA with viability measured at 24 h post oridonin by MTT assay, (G) ROS analysis in cells treated with 40 μM oridonin 72 h after NC or siSLC7A11 transfection. Intracellular ROS was detected after treatment with oridonin for 4 h. *P < 0.05, **P < 0.01, compared to the NC.