Skip to main content
. 2019 Oct 11;26(1):62–72. doi: 10.1177/1753425919879503

Figure 3.

Figure 3.

Sodium phenylbutyrate (4-PBA) reduces SLD LPS–induced p62 accumulation and ccl2 expression. Immortalized BMDMs were treated for 9 h with PBS (control), 4-PBA (1 mM), LPS (1 ng/ml), or 4-PBA+LPS and analyzed for p62 protein and transcript expression as well as ccl2 expression. (a) 4-PBA reduced the accumulation of p62 caused by SLD LPS treatment. Quantified blot data on the right. Blot was quantified as described in the Materials and methods and is representative of at least three separate experiments. At least three separate blots were used for quantification and statistical analysis. **P < 0.01; ***P < 0.001. (b) p62 transcript expression. (c) p62 blot quantification from (a) divided by p62 transcript expression in (b) as described in Figure 2 and in the Materials and Methods. (d) ccl2 transcript expression. Transcript data were obtained as previously described in Figure 1 and as described in the Materials and methods section. Data from at least three separate quantitative RT-PCR experiments were used for statistical analysis and to produce the graph. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.