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. 2020 Jan 21;20:65. doi: 10.1186/s12879-020-4783-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of IL-27 on IL-6 and IL-8 release from hemozoin-exposed peripheral blood mononuclear cells (PBMCs). PBMCs were primed with recombinant human (rh)IL-27 (100 ng/mL, 90 min) and incubated with different concentrations of hemozoin (Hz) ranging from 10 to 200 μg/mL (indicated as Hz10, Hz50, Hz100 and Hz200) for 22 h. IL-6 (a and b) and IL-8 (c and d) was measured in supernatants from the cells with EIA. Data are presented as mean and SEM of three (IL-6 data) and five (IL-8 data) separate experiments. ***p < 0.001 versus unstimulated (US) cells (white bar), and †p < 0.05 and ††p < 0.01 versus Hz (blue bar)