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. 2020 Jan 21;20:65. doi: 10.1186/s12879-020-4783-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effects of hemozoin on IL-27Rα and gp130 gene expression in HAoEC and PBMC. The cells were incubated with different concentrations of hemozoin (Hz) ranging from 10 to 200 μg/mL (indicated as Hz10, Hz50, Hz100 and Hz200) for five (a) and 22 (b-d) hours. Gene expression analyses were done by qPCR, related to reference gene β-actin/TaqMan reference probes and normalized to unstimulated cells (US). The figure shows mRNA levels of IL-27Rα and gp130 in HAoEC (a and b) and in PBMC (c and d). Results are representatives of minimum three experiments and data are presented as mean and SEM. *p < 0.05 and **p < 0.01 versus unstimulated cells (white bar)