Fig. 4.

Localization of Sca-1^high fibroblasts and Sca-1^low fibroblasts in adult mouse lungs. Hoechst 33342 (a), lineage-specific cell surface markers (CD31, CD45, CD146, E-cadherin, LYVE1, and TER-119) (b), Sca-1 (c), and PDGFRA (d) staining, merged images (e). Mouse lungs were also stained with hematoxylin and eosin (H&E) (f) and Elastica van Gieson stain (EVG) (g); collagen fibers are indicated as red bundles, and elastic fibers are indicated as black bundles. Arrows in (a) to (e) indicate Sca-1^highlin^negPDGFRA^high fibroblasts localized in the adventitia of pulmonary artery. Hoechst 33342 (h), lineage-specific cell surface markers (i), Sca-1 (j), and ITGA8 (k) staining, merged images (l). Mouse lungs were also stained with H&E (m) and EVG (n). Arrows in (h) to (l) indicate Sca-1^highlin^negITGA8^low fibroblasts localized in the bronchovascular interstitium. Hoechst 33342 (o), lineage-specific cell surface markers and Sca-1 (p), ITGA8 (q), and PDGFRA (r) staining, merged images (s). Mouse lungs were also stained with H&E (t) and EVG (u). Arrows in (o) to (s) indicate Sca-1^lowlin^negITGA8^high fibroblasts localized in the adventitia of pulmonary vein. Arrows in (u) indicate elastic fibers in the adventitia of pulmonary vein. Br, bronchial epithelium. PA, pulmonary artery. PV, pulmonary vein. Representative results are presented in all panels; scale bars, 50 μm